Japanese beech (Fagus crenata Blume, Fagaceae) is a long-lived woody angiosperm, with an outcrossing breeding system involving wind pollination. This study examined the pollen dispersal patterns of F. crenata in a typical cool-temperate deciduous broad-leaved forest in Japan. Study site was a 4-ha permanent plot (200x200 m). Beech nuts were collected under the crown of 20 adult trees in October 2009. The nuts were divided into pericarps and embryos and DNA was extracted separately. Candidate seed and pollen parents were 322 adult trees. One set of seven microsatellite primer-pairs for multiplex PCR were used to genotype individuals. Four hundreds and eighty-two nuts were used for maternal analysis with pericarp DNA and seed parents were determined in 229 nuts. Paternal analysis using embryo DNA determined pollen parents of 112 nuts out of the 229 nuts. The other 117 nuts were considered that their pollen parents existed outside the study plot. The mean distance between seed and pollen parents was 58.2 m ± 46.9 (SD). The largest and smallest distances between seed and pollen parents were 160.5 m and 2.2 m, respectively. Six nuts were inferred to be self-fertilized seeds. Finally, we will examine the pollen dispersal kernel using the neighborhood model to evaluate pollen dispersal patterns in the species.