We estimated strength of inbreeding depression and selfing rate in L. japonicum var.abeanum and inspected possibility that it experienced high inbreeding depression. We also studied the relation of stigma-anther distance and selfing rate because there was polymorphism in stigma-anther distance. We measured stigma-anther distance after flowering and estimated inbreeding depression and outcrossing rate in parental individuals and seeds by microsatellite markers. As a result, stigma-anther distance is binomial distribution, selfing rate decreased so that stigma-anther distance was large. The average selfing rate of population reached 0.60. However, for strong inbreeding depression such as 0.52, the inbreeding coefficient of parental generation was small as 0.17. The seeds derived from selfing were hard to be produced by inbreeding depression. In L. japonicum var.abeanum, the reserves of deleterious genes are still in high state. It's suggested that result in L. japonicum var.abeanum are exposed to strong inbreeding depression. We considered that it's important that preventing decrease of population size and inbreeding, and fixing condition that pollinators are easy to visit as environment promoted crossing to conserve L. japonicum var. abeanum.