Many studies have suggested that climate change affected phenology of animals and plants. To clarify the effect of climate change on the phenology of plants and animals, it is necessary to use data on temperature and phenology over several centuries. However, such kind of data is quite limited. In this study, we compared the phenological data in the old documents in 17th and 19th Century (Edo era) with that of present days which were collected by the Japan Meteorological Agency. For the comparison, the phenology of 3 plants and 5 animals in Kyoto and 2 plants and 3 animals in Tokyo were selected. ”Climate change model” predict that spring and summer phenology will become earlier and autumn phenology will delay by climate change. The major results were: 1) current phenological changes were larger than in the Edo era, 2) phenological changes were larger for animals than for plants, and 3) the phenological changes of summer and autumn mostly followed the trend predicted by the climate change model, though the spring phenology showed mixed results. By comparing current phenology with that of 1600’s, this study clarified that phenology were influenced by climate change.