For appropriate decision making in ecosystem management for global warming prevention and biodiversity conservation, a reliable and practical method to evaluate ecosystem services is necessary. The research objectives are (1) integration of biophysical and socio-economic data related to ecosystem services (2) development of a practical evaluation method of ecosystem services and (3) contribution to mitigate conflicts between environmental mitigation options such as climate change prevention and biodiversity conservation. For the purpose, we have conducted a pre-evaluation of ecosystem services; climate regulation and reservoir of biodiversity. The study area, Kushiro watershed was mapped using the InVEST, which is ecosystem services mapping tool developed by Natural Capital Project, preliminarily. The analysis conducted based on the land cover maps in 1970’s and 2000s. The change in ecosystem services was spatial-explicitly quantified. The climate regulating service and the biodiversity reserving service were totally degraded, however the change was depends on location due to various land use change happened in the area. Further development is needed to provide a more practical evaluation to communicate with stakeholders.