Arabidopsis halleri subsp. gemmifera is a perennial herb that produces two types of leaves; rosette and cauline. Rosette leaves persist throughout the life and cauline leaves appear during reproductive period. The surface feature of leaves related to wettability was studied in 140 individuals growing in understory (ca. 430m) and semi alpine (ca. 1370m) habitats of Mt. Ibuki. The cauline leaves of understory plants were highly wettable compared to the semi alpine plants and showed significant decrease in leaf wettability with increasing altitudes. However, rosette leaves of both habitats were highly wettable and showed no altitudinal variations. We also conduct control experiment to compare these results. The leaf wettability of cauline leaves under natural and controlled conditions showed similar trend with altitudes. The same pattern was observed for the rosette leaves under both conditions. The overall leaf wettability analysis suggested the existence of two types of leaf wettabilites for the same plants growing in semi alpine habitat; less wettable in cauline leaves and highly wettable in rosette leaves. The consistent results obtained from natural habitats and growth chamber suggested that the difference in leaf wettability is attributable to both environmental and genetic factors.