The significance of aerodynamic controls on tree transpiration (E) is critical when modeling E using the simplified Penman-Monteith equation. However, few studies have explicitly examined the difference in the significance of aerodynamic control on E from leaf shape. We hypothesized that the significance of E regulation by w (wind speed) is difficult to identify from leaf shape such as conifer-type needles or broadleaf leaves and that the response of E to w could be significant even in conifer species. We performed sap flux measurements using a wind tunnel on two conifer species: Chamaecyparis Obtusa var. formosana, and Araucaria cunninghamii; and three broadleaf species: Swietenia mahagoni, Michelia compressa, and Plumeria rubra. C. obtusa and P. rubra showed no significant E response to w. A. cunninghamii, S. mahagoni, and M. compressa showed a significant increase in E to w, supporting our hypothesis. Our experimental and numerical simulation data suggested the dependency of aerodynamic controls on E probably results from the differences in stomatal conductance (gs) rather than leaf shape. We emphasize the importance in confirming the significance of aerodynamic controls on E using both direct and/or indirect measurements.