Species richness and species composition of microfungi associated with Oregon beaked moss (Kindbergia oregana) were studied at two forest chronosequences on southeast Vancouver Island, British Columbia, Canada. The purposes were to investigate the effects of clear-cutting and the transformation of old-growth forests into secondary forests on microfungi and the succession of microfungi in relation to long-term stand development. Green and brown parts of moss were collected from the forest floor of Douglas-fir stands of four age classes: post-harvest regeneration (13-14 yrs), immature (50-51 yrs), and mature (85-101 yrs) stands, and a control old-growth (296-324 yrs) stand, and used for the isolation of fungi. A total of 49 fungal species were recorded. Study site, stand age, and moss parts significantly affected the species richness and species composition of fungi. The species richness of fungi was significantly greater on brown than on green moss parts and lower in post-harvest regenerations than in forest stands of the other age classes. The species composition of major fungal species changed gradually along the seral stages.