Whether lizard species in various thermal environments maintain similar thermal profiles and thermal performance curves has been debated. In this study, we compared the thermal characteristics between two populations of Japalura swinhonis from lowland and mid-altitude. We measured lizard sprint speed at 6 temperatures to calculate performance breadth (B₈₀) and thermal optimum (T_o). We also measured thermal tolerance limits (CT_max, CT_min), preferred temperature (T_sel) and field body temperature (T_b).We found that B₈₀ in middle altitude population is significant wider than that of lowland population ; B₈₀ of female is significant wider than that of male. T_o, T_b and CT_max in mid-altitude population is significant lower than lowland ones. Thermal tolerance range in male is significant wider than female. There is no significant difference in T_sel between two populations or two sexes. Our results revealed that J. swinhonis has different thermal profiles and performance curves in different altitudes, supporting the labile view on the evolution of thermal physiology for lizards.