Wolbachia is a reproductive parasite in a variety of arthropods including at least 20% of all insect species. Although Wolbachia normally undergoes vertical transmission through the maternal line of its host population, there is compelling evidence from molecular phylogenies that widespread horizontal transmission must have occurred. To investigate host specific difficulty in horizontal transmission, we artificially emulated the parasitoid-mediated transmission by injecting Wolbachia taken from Callosobruchus analis to the abdominal cavity of female pupae of the following two types of recipients: C. analis treated with antibiotics for eradicating Wolbachia and C. maculatus being inherently Wolbachia-free. A zero-inflated Bayesian analysis showed that Wolbachia transmission rate was higher for C. analis than C. maculatus recipients. Thus, Wolbachia was horizontally transmitted more easily to its native host than novel hosts. But the transmitted Wolbachia in the recipients could not get into the host germ line in both C. analis and C. maculatus. Horizontal transmission of Wolbachia was severely prohibited by multiple barriers of host species.