Holoparasitic plants lack chlorophylls and gain nutrients and water for growth from host plants. The physiological difference between holoparasitic and photoautotroph plants would cause different demand for nutrients. Balanophora laxiflora, a holoparasitic plant, commonly distributes in Taiwan. Most of previous studies worked on its medicinal ingredients. Our research focus on nutrient relationship between B. laxiflora and its host plant, Elatostema lineolatum var. majus. In order to understand the source and nutrient contents in B. laxiflora, we analyzed its carbon and nitrogen contents, stable isotopes and other mineral nutrients (phosphorus, calcium, magnesium, potassium, and sodium) content. The results indicated that δ¹⁵N values of the parasite were significantly higher than its hosts. The δ¹³C values were not significantly different between parasite and host, a positive correlation between the value of δ¹³C of parasite and those of host plants was found. It indicated that δ¹³C might be used to trace the nutrients source of the parasite. The elemental analysis revealed parasites had significantly higher carbon, potassium and sodium contents than host plants. The higher contents of potassium and sodium may associate with regulation of water potential in holoparasitic plants.