Isoprene is a volatile organic compound (VOC) with high volatility and reactivity. Currently, isoprene emission is essential information for modeling of O₃ and CH₄ dynamics in atmosphere. Moso bamboo, which has been reported with significant isoprene emissions, is a dominant component of forest area in eastern Asia. The area of moso bamboo has increased in Japan; furthermore, the expansion would be faster under the context of global warming. Therefore, there is an urgency to evaluate the isoprene emissions from bamboos. However, currently, the temperature dependence of isoprene emission flux from moso bamboo which is essential for evaluations is still unclear. To clarify the temperature dependence, this study observed isoprene emission fluxes from leaves of moso bamboo with light control during measurements in a pure moso bamboo forest of central Taiwan from September 2015 to March 2016. Under sufficient light, the seasonal changes in the isoprene emission fluxes were mainly increase with leaf temperature, where leaf temperature ranged from 15 to 27 °C, and isoprene emission flux ranged from 0-53 nmol m^-2 s^-1. Very low or no isoprene emission fluxes were found in leaf temperatures < 23 °C, causing misestimation by a widely-used isoprene emission model.