Lailati et al (P1-462) report effects of bamboo chip mulching on rice production. In this study, we evaluated effects of bamboo chip mulching on paddy field biodiversity using Odonata as an indicator group. We conducted an experiment using 16 experimental paddy fields (each 30 m2). We established five treatments with three to four replicates each: 1) control, 2) manual weeding, 3) low-volume bamboo chip application (0.5t/10a), medium-volume bamboo chip application (1.0t/10a) and high-volume bamboo chip application (2.0t/10a). From July to September, we carried out weekly odonate exuviae sampling at each paddy field. We also performed environmental DNA (eDNA) analysis twice when Odonata emergence rates were high. The results from exuviae sampling showed that taxonomic richness was lower in the high-volume bamboo chip application treatment relative to other treatments. Damselfly emergence was higher in the low- and medium-volume bamboo chip application treatments relative to controls. In contrast, Sympetrum dragonfly emergence was not observed from rice paddies with medium- and high-volume bamboo chip application. These results suggest that applying large amount of bamboo chips has detrimental effects on Odonata diversity and bamboo chip mulching has contrasting effects on damselflies and Sympetrum. If possible, eDNA results will also be presented at the conference.