Desertification cause serious environmental problems in Northern-east Asia and China is one of the most serious countries where damaged by desertification. Vegetation restoration is considered as a effective way to recover desertification area as original state. However, grazing of livestock can reduce restoration effect in restoration area because restored plants may be useful dietary food sources for livestock. In order to determine which plants can be grazed by the livestock in restoration sites, we investigated grazing preference of livestock from fecal samples based on DNA meta-barcoding with high-throughput sequencing(HTS). We collected 77 feces from restoration areas and one pine forest as reference sites in Hulunbuir steppe. Total 84 different molecular operational taxonomic units (MOTUs) were detected from feces. Relative read abundance (RRA) information represented that four plant species (Onobrychis sp., Caragana sp., Corispermum sp., Carex sp.) were identified as major foraged species and comprised large proportion. The diet composition of livestocks in restoration regions differed significantly with reference sites, but there were no significant difference between livestock species. Caragana sp. which planted for restoration can be one of the most preferred dietary sources for livestock than other planted species. We expected that our results provided useful information for establishment of restoration strategies in desertification area.