Understanding temporal dynamics and ecological stability allows us to quantify how ecosystems respond to and recover from disturbance. Here, I give a brief overview of the importance of considering temporal aspects of ecological stability. I present a case study from the world’s largest ecoacoustic dataset, collected as part of the OKEON (Okinawa Environmental Observation Network) project, a scientific monitoring network across 24 sites in Okinawa. Despite increasing recognition of the potential of acoustic monitoring, there remains a dearth of ecoacoustic studies testing macroecological hypotheses, and no methodological precedent for using acoustic data to measure ecological stability. I calculated the diversity and variability of the biotic soundscape using two opposing methods. I found that existing methods resulted in an increased probability of Type 1 error, and a weak negative diversity-stability relationship. I present a method to calculate ecoacoustic variability independently of diversity and find evidence for a strong, positive ecoacoustic diversity-stability relationship when using this method. This study reveals the challenges of using sound data to test ecological hypotheses but highlights the utility of remote acoustic monitoring practices in providing a holistic picture of the temporal dynamics and stability of ecological communities and their resilience to global change.