Genetic diversity is one of important components of biodiversity, and its conservation and management are beginning to attract attention at the scenes of breeding or tree planting activities. In this study, we attempted to make a simulation program that estimate how genetic parameters (expected heterozygosity and effective numbers of allele) of collected seeds change along with the change of the numbers of collected seed and that of seed source trees.

The simulation was carried out using genotype data of 198 Fagus crenata mature trees in a population and 653 seeds collected from 4 mother trees. Mother trees were randomly selected within a population, and seeds were generated to ensure that pollen pool diversity in simulation corresponds to average of observed one from 4 mother trees. Although genetic parameters in seed population increased when numbers of seed or numbers of mother tree increased, they reached a ceiling when number of mothers was more than ten in our dataset. Our program will be one of useful tool to estimate the effect of sampling design on the genetic diversity of collected seeds.