Bidens pilosa var. radiata is a perennial and invasive plant in Taiwan. After removing all plant parts in a farm dominated by B. pilosa var. radiata, we found that this species dominated again. Thus, we suggest to plant suitable species that prevent the regrowth of B. pilosa var. radiata after its removal. Two native perennial species of Taiwan, Sambucus chinensis and Urena lobata, were selected. We compared seed germination of the three species under two light regimes and examined effects of aqueous tissue extracts of the native species on seed germination and seedling growth of B. pilosa var. radiata to evaluate the effectiveness of the two native species as the inhibitor of the growth of B. pilosa var. radiata. Preliminary results showed that seeds of U. lobata germinated fastest and had the highest germination percentage in both light treatments. Seeds of S. chinensis germinated slowly and only 37% (shading) and 52% (control) germinated after 2 months of treatments. Seed germination and hypocotyl elongation of B. pilosa var. radiata were significantly reduced by stem extract of S. chinensis and that of U. lobata. In conclusion, U. lobata may be more suitable than S. chinesis for preventing the regrowth of B. pilosa var. radiata.