Headwater catchments are important for water and nutrient supply regulation to downstream.  However, rapid land use land cover change has affected the hydrological functions of the tropical water catchments.  In this paper, we analyze the impact of land use change on annual and seasonal water yield for 5 selected water catchments in Peninsular Malaysia.  The land use change in the catchments are assessed through land use map of year 2000 and 2008. Monthly water yield for 2000 and 2008 are simulated by applying the InVEST water yield model using observed precipitation, land use, soil depth, evapotranspiration, and plant available water fraction data. Land use change analysis shows scenarios of land use change including forested land conversion, expansion of urban land, switching of others crop land to oil palm. Statistical model R2 assessment indicate good agreement between observed and stimulated water yield. The stimulated monthly water yield show significant shift in annual yields. But, rainfall distribution is found to be the dominant effect in annual water yield. While land use change has minor impact on the annual water yield which resulted slightly increase in water yield. Conversely, finding shows moderate impact of land use change in seasonal water yield as the water yield in 2008 show significant decrease during dry season due to expansion of agricultural land.