Myanmar’s mining sector is mainly an artisanal industry producing small amounts of copper, gold, lead, nickel, tin, tungsten, and zinc (USGS, 2014). Due to the lack of information and technique, there are many environmental problems causing around mining area. In this study, the total Hg concentration of soil, rice, vegetables, fish, hair, water, and atmospheric concentration around the ASGM area, Thabeikkyin Township were investigated. Mercury concentrations in the samples were measured by heating vaporization method which was detected as total Hg and the detection limit was 0.002 ng (MA-2000, Nippon Instrument Cooperation). The total Hg concentration of air, soil, and vegetables showed high concentrations and the values were over the limit level of the standard values which is recommended by WHO. The total Hg concentration of fish ranged from 0.02 to 1.01 μg/g so that some of the fish had the higher concentration than the WHO recommendation. Moreover, the total Hg concentrations of water samples ranged from 0 to 29.3 ng/l. The mean of Hg concentration of the mine workers and non-mine workers were 1.28±0.84 μg/g and 1.08±0.52 μg/g respectively. Otherwise, the total Hg concentrations of hair levels in mine-workers were higher than the non-mine-worker.