Bitter taste plays an important role in avoiding ingestion of toxins and resisting bacteria and parasites, which might evolve to reflect species-specific diets. Bitter taste receptors (TAS2Rs) mediate bitter perception in mammals. We investigated the polymorphism of a bitter taste receptor TAS2R38, protein for the bitter PTC, in four allopatric Macaca species (M. hecki (N: 16), M. tonkeana (N: 12), M. nigrescens (N: 11) and M. nigra (N: 15)) in Sulawesi Island, Indonesia. We observed all individuals of M. hecki are sensitive to PTC while some individuals showed low sensitive to PTC in the three species. Determining TAS2R38 sequence, we found truncated TAS2R38 led to no sensitivity of PTC in M. nigra and M. nigrescens. In vitro, we confirmed substitution on three amino acid sites are responsible for low sensitivity in M. tonkeana. we found TAS2R38 of Sulawesi macaques is derived from M. nemestrina and the low-sensitive allele in M. tonkeana is shared with M. nemestrina. The non-sensitive alleles occurred independently in M. nigra and M. nigrescens after speciation. The intact low-sensitive alleles may respond to other bitter compounds. These results revealed species difference on bitter taste; however, whether these differences were resulted from local adaptation need to be studied.