We compared natural sound recordings from 24 field sites across Okinawa as part of the OKEON 美森 Project, focusing on the dawn chorus in the spring breeding season. We used high performance computing to process > 50 TB of environmental audio during the period of 2017-2018. We found that distance to buildings was the best predictor of both the presence of animal noise and the absence of human noise. Urban areas, and especially those close to the coast, tended to be noisier and have weaker and less diverse dawn choruses. Interestingly, these more disturbed locations tended to exhibit greater acoustic beta-diversity compared to Okinawa’s inland forests, which differed little from one another. Our results suggest that anthropogenic disturbance alters not only the physical environment but also the sensory environment that animals inhabit.