Many animals can navigate toward their destination, and this ability is observed at different spatio-temporal levels. Navigation has been studied, both behaviorally for wild animals, such as seabirds migrating long distances, and neurophysiologically for laboratory animals, such as rodents. However, there have been considerable discrepancies between the results of the field- and laboratory-based navigation studies due to the technological difficulties in applying neurorecording techniques to wild animals. In this study, we have recorded the neural activity of the freely-moving seabirds, streaked shearwaters Calonectris leucomelas, on Awashima Island, Japan (38°28′N, 139°14′E), using the recently developed wireless neurologgers. For this purpose, we established a surgical method for inserting electrodes into the brains of the seabirds. we also developed a perfusion fixation and antibody staining method for the seabird brains after the neurorecording to confirm the site at which the neurons were recorded. As a result, we discovered the navigation-related neurons that have been reported in mammals.