A promising tool- genome-wide field transcriptome analysis is in a combination with relative gene expression analysis, by comparing and analysis the seasonal changes of carbohydrates, nitrogen and phosphorus transport in leaves and buds of two evergreen masting species, to explore roles of these transporters in the metabolic coordination network between source leaves and sink bud organs to respond to seasonal climate change and their relationships with phenology of evergreen trees.

How transporter genes are expressed in natural seasonal environments is rarely studied. We here investigate seasonal expression of transporter genes associated with nitrogen metabolism, sugar, and phosphorus transport about two years in natural conditions using two evergreen tree species, and which results showed nitrogen metabolism transport genes are highly expressed in summer and winter, while sugar and phosphorus transport genes are highly expressed in winter .

In these biological processes, we chose five candidate genes for the further verification of RT-qPCR experiment in leaves at three different study sites underlying gene function.
We compared the differences between the candidate genes in the same species at different sites, and differences between the two species at the same site, to have a further understanding of nutrient status effect on tree’s phenology.