Gammaridean amphipod (Crustacea: Amphipoda) is a major component of marine benthic community and is known as a good bioindicator of environmental stresses. However, information on their distribution and ecology is still limited in subtidal zone of northern Japan. The present study aimed to clarify the species composition and community structure of benthic amphipods in subtidal shallow bottoms of Akkeshi Bay, eastern Hokkaido, in relation to environmental gradients. Field collections were conducted for 9 sites mainly in the spring of 2020. Amphipod species belonging to 21 families and 39 species were identified. Most species have been reported arctic - subarctic distribution, and six species are considered new to Japanese record. Similarity analyses revealed that the amphipod community differed among three habitats; soft-bottom, hard-bottom, and estuary, and that the soft-bottom community was subdivided into three subgroups. For the soft-bottom community, species occurrence pattern was well explained by the variation in benthic water temperature and salinity. In conclusion, the present study showed that the amphipod community in Akkeshi is affected by subarctic attributes, and that the community structure is influenced by benthic parameters in soft bottom habitats such as bottom water characteristics.