In a tropical rain forest, multilayered structure is formed by the mixture of various tree species and organisms at different growth stages. Therefore, leaf litter locates in many positions, such as above the branches. However, the decomposition process, including soil animal influences, is discussed almost about the litter on the forest floor. Hence, the aim of this study is to assess the differences in decomposition rate and the impact of soil animal at various locations in a tropical rainforest in Malaysia. Litter bag experiments were conducted at the following five locations, on soil, on litter, on fallen trees, at the base of palms, and at the branches in the air. Two types of litter bags with different mesh sizes, fine and coarse, were used at each location to evaluate the effects of soil animals. We used filter paper and cellulose sheets as standard material and leaf litter randomly collected from the forest floor. We found that the decomposition of litter captured by branches was the slowest. Soil animal impacts were particularly large on litter and on fallen trees in the standard material. Whereas, in leaf litter, the impact of soil animals was similar at all locations.