Fossils encompassing multiple individuals provide a rare opportunity to unveil behavioral interactions among extinct organisms. However, a fossilization process can affect the spatial relationship between individuals and thus interfere with the accurate reconstruction of the behavior. Here, we developed an empirical approach combining fossil evidence with taphonomical experiments to estimate how behavior preserved in amber fossils reflects the behavior before trapping. To simulate natural entrapment by tree resin, we used a sticky trap and observed how the tandem running behavior of termite mating pairs changes upon entrapment. Our experiments demonstrated that a pair on a sticky surface has a unique spatial positioning, distinct from natural tandem running pairs. Followers were always behind the leader in natural tandems, while pairs on a sticky trap were aligned side-by-side and had closer interindividual distance. As a fossil reference, we leverage an unprecedented Baltic amber syninclusion of female and male Electrotermes affinis. Our quantitative spatial analysis shows that the inter-individual position of the termite mating pair in the fossil is consistent with that of the experimentally entrapped termite tandems. The inference of behavioral interaction among extinct animals thus can be refined through taphonomic behavioral experiments using modern relatives of extinct organisms.