Social insects form a well-organized society characterized by a division of labor, where colony members perform various tasks within different nest areas. Within their nests, a wide range of species known as guests, exploit resources from the host colony. To comprehend these exploitation strategies, it is essential to consider spatial heterogeneity within the nest, as the type and amounts of resources available to guests vary across locations such as royal chambers, foraging areas, and brood chambers. In this study, we demonstrated that the rove beetle Trichopsenius japonicus, a guest species of the termite Reticulitermes speratus, distributes in the royal chamber, where it has increased opportunities to receive food supplies from the hosts by accompanying the termite queen. First, the investigation of the distribution of rove beetles within natural termite colonies revealed that they are concentrated in and around the royal chambers. Laboratory experiments showed that the rove beetles were more likely to receive provisioning from workers around the royal chamber compared to those from other areas. Furthermore, the rove beetles were attracted to the location of the queens and increased their begging to workers when in the presence of the queens. These findings suggest that rove beetles have developed strategies to efficiently acquire provisioning from their hosts by using the presence of queens as a cue for positioning themselves in the royal chamber. Our results also provide important implications for task allocation among workers, as we identified differences in the responses of workers distributed around the royal chamber and other areas to the begging by rove beetles. This study sheds light on the complexity of interactions between social insects and their guests, which is dependent on spatial variations in host behavior and corresponding guest strategies.