Tire Particles (TP) are a growing environmental and health concern because of their widespread distribution and potential toxicity. Every year around 1.4 million tons of TP are released into the environment, 67% of which ends up in soil. The composition of TP is complex, consisting of various components like 48% of polymers, mainly styrene-butadiene rubber, 22% of filers (carbon black), 15% of metals, 5% of fabrics, and 10% of other compounds. Antioxidants such as 6-PPD, BTZ, DPG, HMMM, melamine, CPPD, and IPPD to improve the tire performance and quality. These particles are deposited on agricultural land or via stormwater runoff, they enter nearby water bodies close to farmland. Many farmers use this water for irrigation, which can lead to the contamination of agricultural systems with TP leachate. Their leachable compounds and chemicals are subject to potential risks for phytotoxicity. Research has shown that TPs may change soil characteristics, including water retention and nutrient availability; however, the impact on plant growth and survival is unknown. This study investigates the effects of tire leachate on the growth and development of Rice (Oryza sative), focusing on their physical and biochemical impact. Rice is the main staple crop and the main source of food for almost half the world's population is cultivated worldwide in common and hybrid varieties. Experiments were conducted to evaluate the toxicity of Oryza sativa seed and seedlings exposed to various concentrations of tire leachate to analyze their physiological and biochemical responses. The Germination index shows that the seeds were able to grow even in the high concentration of TP leachate. The tough and resilient seed coat protects the seed from environmental pollutants. In addition, the seedlings showed increased growth at higher concentrations of TP leachate than in the control group. This suggests that the leachates from the TP contain certain nutrients that stimulate the growth of paddy.