The increase in human populations and demand for agricultural goods have led to the conversion of forest reserves into agricultural plantations for agricultural activities, resulting for habitat loss. These land-use changes have led to mammal extinction threats and becoming highly vulnerable to activities (habitat destruction/ fragmentation). This study addressed the occupancy and diversity of mammals in tropical forest reserves and agricultural plantation landscapes (orchard, oil palm and rubber plantations) in Negeri Sembilan, Malaysia. Using camera-trapping method, this research ought to understand how different landscapes support varying levels of biodiversity and influence the occupancy of species in the locations. To find the relationship between mammal occupancy and environmental factors, we assessed (1) tree abundance within 50 m x 50 m plot surrounding sampling points, (2) tree height, (3) understory vegetation cover, (4) understory vegetation height, (5) distance to forest, (6) standing snag and (7) fallen snag were taken. A total of 18,604 photographs were captured with the highest in orchard (5,291) followed by rubber (5,424), oil palm (5,291) and forest (3,306). Forest reserves exhibited the highest mammal species richness with 14 species followed by orchard (6), oil palm (5) and rubber (5). From the findings, species occupancy were found to be significantly affected by few factors such as elevation, distance to forests, the presence of fallen/standing snags, and tree abundance. The results highlighted the importance of maintaining different habitat types, including less disturbed forest and modified landscapes with natural elements, to support mammal diversity. This study may contributes to understand factors influencing mammal occupancy and help balance conservation efforts with economic demands in the context of ongoing land-use change in Southeast Asia.