Phanerochaete velutina, a wood decay fungus, forms a mycelial network several meters scale on the forest floor. When the mycelium contacts wood baits, active branching occurs not only at the location of contact (stimulated sites),but also at the location without contact (non-stimulated sites). This phenomenon suggests the signal transduction within the mycelial network. However, the existence of signals has not been verified, and the branching at non-stimulated sites, which is thought to be caused by signals, has not been quantitatively verified. In this study, we quantified the number of hyphal tips in the mycelial network to statistically verify branching at non-stimulated sites on soil medium. The number of hyphal tips was determined by software analysis of mycelial images obtained from experiments in which mycelium was stimulated by wood (bait), acrylic (contact), and no stimulation. The increase rate of hyphal tips from day 1 to day 6 after the stimulation in each soil plate showed that the rate was significantly higher in the bait experiment compared to the other conditions. In addition, linear elongation of hypha was observed in both stimulated and unstimulated sites in contact experiments. These results suggest that the existence of a branching signal specific to the bait experiment and a linear elongation signal specific to the contact experiment. Since some of the stimuli cause both changes in electrical potential and Ca²⁺ concentration in wood decay fungi, we hypothesized that Ca²⁺ is involved in signaling to non-stimulated sites in this species. Therefor we attempted to generate transgenic individuals of this species that visualize intracellular Ca²⁺ in response to stimuli by expression of Ca²⁺ marker protein. For which genome analysis and recombination have been unprecedented in this species, we have revealed the whole genome sequence, high-expressing promoters, codon frequencies, and the effectiveness of the Agrobacterium method for the first time.