Anthropogenic disturbances and climate change pose a significant challenge for the long-term health of forest ecosystems. Many forests are in various stages of succession and are facing additional stresses such as forest fires and invasive species due to human activity. These additional stresses necessitate active human intervention to help maintain forests and support their functions. As forest management becomes increasingly necessary to mitigate climate change, it is important to consider the effects of forest management on soil microbial ecosystems. The soil biota provides many functions that support plant function and help abate climate change such as sequestering carbon and cycling nutrients. As such it is important to consider how long-term human ecosystem interventions may impact the structure of belowground microbial communities. While there is a general understanding of how forest management impacts ecosystems above-ground, there are still considerable gaps in our understanding on how below-ground soil microbial communities are impacted, especially in the long term. To fill this gap, we conducted an experiment to see how bacterial and fungal communities differ between long-term managed sites and naturally vegetating forests. An assessment was made on previously established sites located in Mitaka City, Japan. These sites have been annually cleared of their undergrowth or left to naturally vegetate without human intervention for over twenty years. Soil samples were taken seasonally and processed using 16s and ITS amplicon sequencing. Soil bacterial and fungal communities were examined and compared to assess how forest management alters soil community structure. Through this experiment, we assessed how forest management alters soil community structure, the drivers for alteration, and the implications this might have for ongoing sustainability initiatives.