Mating opportunity conditions often involve evolutionary change due to male manipulation during mating as an attempt by the male to assure paternity. In an eradication program of the sweetpotato weevil Cylas formicarius in Okinawa, Japan, the weevils have been mass-reared artificially for over 90 generations for using a sterile insect technique. In this weevil, females do not secrete the sex pheromone after her first mating in the field condition. However, under the high-density such as the mass-rearing conditions, females might mate multiply because given many chance to mate by many males after first mating. The manipulation traits of mass-reared males might have been evolved; therefore we examined the effect of the mating for mass-reared males on female. In the results, we found that the female mated with some of the mass-reared males die early. Furthermore, we also found that the ejaculated semen of some mass-reared males led to female short-lived by injecting male genital derived substances into virgin female abdomens. We discuss adaptive significant and paternity of these traits with the males led to early female death.