Group-living animals evaluate alternaive nesting sites before nest-moving. Presence of competitors around nestling sites is also one of nest qualities relevant to nest selection. House-hunting ants avoid the nesting sites near which other conspecific ants colonize and the nests where chemicals secreted by non-nestmates remain. Here, we investigated how parthonogenetic ants, Pristomyrmex punctatus, respond to chemical traces of conspefic and heterospefic ants. This ant is suitable for nest-moving experimetns, since this ant moves nests frequently, and it is not difficlut to reconstruct habitats of this ant under laboratory conditions. Two-chicoe tests revealed that ants preferred a nesting site with secretions of non-nestmates to a clean nesting site. A nesting stite marked by nestmates was also seleceted more frequently than a clean nesting site. When nesting sites marked by nestmates and non-nestmates were presented, both sites were seleceted evenly. These results indicated that P. punctatus had differetnt nesting strategy from house-hunting ants. Chimcal traces of different colonies would inform the presence of competitors for house-hunting ants, but usability of nesting sites for P. punctatus. P. punctatus also selected nesting sites marked by heterospecific ants as frequently as clean nesting sites and nestmate-marking sites.