This study aims to investigate safe strategy to control <Aedes aegypti/> using the most potential <Bacillus thuringiensis/> isolated from East Java as a natural enemy. The study was carried out the isolation of <B. thuringiensis/> around East Java and it conducted a toxicity test. The results of this study showed that all locations of East Java have a larger House Index (HI) than the WHO standard for the high dengue hemorrhagic fever (DHF) risk area. The incidence rate of DHF was affected by the maximum and minimum air temperatures,mass of rainfall, and wind velocity. The predominant mosquito in their community found in East Java was <Aedes aegypti/>. <Bacillus thuringiensis/> isolated from East Java more effective than <Bacillus thuringiensis/> var.<israelensis/> to control <Aedes aegypti/> larvae. <Bacillus thuringiensis/> isolated from East Java has no effect against <Trichogaster pectoralis/> of a toxicity test. This fact indicates that <Bacillus thuringiensis/> indigenously isolated from East Java has a high potential natural enemy to control of <Aedes aegypti/>. The most important finding about the biological control effort is the usage of natural enemies where they come from the same habitat as disease vectors.