Patterns of signal divergence can provide insight into evolutionary processes of communication system. The forces undergo signal divergence are selection such as interaction between species, genetic drift and recently proposed cultural drift. We tested hypotheses: reproductive character displacement, genetic and cultural drift to explain geographically acoustic variation of M. fissipes in Taiwan. We recorded calls from 11 populations, including 8 sympatric population distributed with closely related M. heymonsi. Genetic and cultural drift were tested by examining the correlation of acoustic differences between genetic and geographic distance. Results showed no significant tuning of calls in sympatric populations. Population structure constructed by COI demonstrated that M. fissipes in Taiwan can be divided into three clades: southwest, south and east, while acoustic signals were significantly different between southwest and east. Acoustic variation was correlated with geographic distance but not genetic distance. To be concluded, geographic variation in advertisement call of Taiwan M. fissipes was caused by cultural drift more than RCD and genetic drift.