It is commonly considered that plants control decomposition processes mainly through litter properties. Recent studies show that plants excrete root exudates, which can accelerate decomposition to acquire N to meet growth demanding for N. Two groups of paired deciduous vs. evergreen tree species from Ilex genera (AM) and Quercus genera (ECM) were used in this study to test species effects on rhizosphere N mining at Yamashiro Experiment Forest,Kyoto Prefecture. Rhizosphere activity can be distinguished both by life form and mycorrhizal types among the species involved. Deciduous species had significantly higher root exudate rates, which lead to a higher proteolytic enzyme activity in rhizosphere soil. Quercus species had an overwhelmingly higher phenolytic enzyme activity than Ilex species in rhizosphere. This effect may result from the oxidation ability of ECM fungi. Although rhizosphere effects varied among life forms and mycorrhizal types, N-related enzymes demonstrated similar responses to root exudates. This suggests that a similarity exists in microbial community in the rhizosphere among different tree species.