Although knowledge of ecological and evolutionary impacts of alien species on native species is critically important for management of biological invasion, determining such impacts can be challenging. Last year, we found hatchlings of alien Bufo japonicus formosus toad in Hokkaido were lethally toxic to native predatory amphibians, larvae of Rana pirica and Hynobius retardatus. Here, to gain insight into impacts of toxic toad on predators in Hokkaido, we compared individual susceptibility and its components (i.e., consumption tendency and toxin resistance) of predators in Hokkaido with congeneric species distributed in natural range of the toad, Honshu (larvae of R. ornativentris and H. nigrescens). We also examined intraspecific variation in susceptibility by using predator individuals from multiple populations. Although mortality of predators exposed to a toad (i.e., susceptibility to the toad) was almost 100% in all R. pirica populations and ranged from 14 to 90 % in H. retardatus populations, it was almost 0% in all populations of predators in Honshu because of difference in toxin resistance. The results raise strong concern about the potential threat of the alien toad. In the poster, we also discuss about evolution of the prospective counter trait of predators in Hokkaido.