Halophytes are main dominant plants in arid saline area of the world. Responses of plants to abiotic stress include the synthesis of osmolytes, which are able to compensate high osmotic pressure without interfering with plant metabolism, even at elevated concentrations. In this study, accumulation inorganic and organic osmolytes were investigated in three kind of halophytes (Crinohalophytes, Succulent Euhalophytes and Psueduhalophtes) growing around Aiding Lake, XinJiang, China. The results obtained showed the succulent euhalophytes accumulated a large amount of Na⁺, whereas low Na⁺ concentrations were observed in the other species. For organic osmolytes, Psueduhalophtes showed high concentrations of soluble carbohydrates, mainly sucrose, and accumulated a large amount of mannitol. Succulent Euhalophytes greatly accumulated glycine betaine. Only Crinohalophytes accumulated γ-butyro betaine with a high concentration. These findings indicate that any particular species normally accumulated at least one type of osmolyte with significant amounts to involve osmotic adjustment in these halophytes. Thus, those halophytes can preserve or enhance their metabolic abilities via osmoregulation under grown arid saline environment.