Calling songs serve an important reproductive function in cicadas. Although every cicada species has its own specific calling song, the calling songs of male cicadas show considerable intra-specific variation. In our previous studies, we found that most of the acoustic variation in Mogannia formosana calling songs was associated with frequency parameters among populations on the island of Taiwan, and the sound frequency of the last short echeme was significantly correlated with individual body size in a chorus of the Green Island population. In this study, we investigated the hypothesis that the frequency elements in calling songs of M. formosana serve as cues for female mate choice by performing three playback experiments of two-choice tests in indoor situations: (1) chorus highest frequency songs vs. chorus lowest frequency songs, (2) species highest frequency songs vs. 2 kHz above species highest frequency songs, and (3) species highest frequency songs vs. tonal band sounds. Our results demonstrated that females had no significant preferences with regard to the song frequency in all three experiments. We suggest that song frequency may be the most important factor for cicada mate choice in long range but not in short range as shown in our playback experiments.