The composition of host-associated microbial communities can have major implications for host health and development. The structure of these communities, like other ecological communities, can be impacted by the colonization order of taxa through priority effects. For embryos developing in bacteria rich environments, such as amphibian embryos, stochasticity in bacterial colonization during development may be particularly impactful. To examine the potential impact that colonization order may have on amphibian embryos, we selectively inoculated treefrog embryos with bacteria isolated from the skin of wild, adult frogs. We inoculated embryos with an inoculum of a single bacterial isolate on the first day, followed by a second inoculum consisting of either a single isolate or a community inoculum of five bacterial isolates on the second day. To understand how the impact of priority effects may differ between host and non-host environments, we set up a mirror experiment that utilized agar in place of developing embryos. Through 16S rRNA amplicon sequencing, we found that the initial pools of bacteria that embryos are exposed to can shape bacterial communities at later life stages, but that the magnitude of this impact is dependent on the identity of colonizing bacteria. Additionally, the impact of priority effects differs between host- and non-host environments, suggesting that the host system also acts to influence bacterial relative abundances.